Abstract: Wireline pumping and sampling technology is usually used to obtain reservoir fluids from offshore exploration wells. However, ultra-low permeability gas reservoirs have complex pore structures and low permeability, and measured-pressure fluid mobility mostly falls below 1.0 mD/(mPa·s). Therefore, conventional pumping and sampling technology is ineffective and challenging to obtain effective formation fluid, which struggles to meet the operation requirements. To address this, the sampling probe module was optimized, and a series of technologies were studied, including structural modification of large sample chambers, module configuration optimization, and simulation analysis of pump pressure recovery in ultra-low permeability reservoirs. Thus an “intermittent pumping and air cushion combined operation” sampling technology was developed. Field applications have demonstrated that this technology effectively enhances sampling success rates in ultra-low permeability gas reservoirs while reducing single-point sampling time and improving operational efficiency. The intermittent pumping and air cushion combined sampling technology provides technical support for reserve evaluation and economically efficient development of offshore ultra-low permeability gas reservoirs.